The HTNV Gn/Gc mutations do not alter glycoprotein colocalization. Human osteosarcoma U2OS cells, transfected with plasmids expressing WT, I532K, S1094L, or I532K/S1094L variants of HTNV Gn/Gc, were fixed at 24 h posttransfection, permeabilized, and coimmunostained with HTNV Gn- and Gc-specific antibodies (see Materials and Methods for details). EV, empty vector. Representative images from a single experiment, out of at least three experiments are shown. Bar, 20 µm.