FIG 1.

The NLRP3 inflammasome is dispensable for Toxoplasma-induced Lewis rat macrophage cell death and IL-1β secretion. Lewis rat BMDMs with or without pretreatment with either 50 μM VX765 or 10 μM MCC950 for 2 h were subjected to infection with Toxoplasma type I (RH) parasites (MOI = 0.5) for 24 h. Macrophage viability was measured via 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Data are displayed as paired scatterplots (left; n = 3; *, P < 0.05; ns, not significant [Student’s t test]). The right scatterplots show the cell viability difference between infected BMDMs with and without treatment in each paired experiment. Horizontal bars represent the median cell viability difference.