TABLE 3.
Primers and plasmids used in this study
| Oligonucleotide or plasmid | Sequence (5′–3′) or notes | Reference |
|---|---|---|
| Oligonucleotides | ||
| Quantitative real-time PCR | ||
| agrB FWD | GACCAGTTTGCCACGTATCT | This study |
| agrB REV | GCTAAGACCTGCATCCCTAATC | This study |
| RNAIII FWD | GGAGTGATTTCAATGGCACAAG | This study |
| RNAIII REV | GTGAATTTGTTCACTGTGTCGATAA | This study |
| psmα1 FWD | GGTATCATCGCTGGCATCATT | This study |
| psmα1 REV | CCATGTGAAAGACCTCCTTTGT | This study |
| pmtA FWD | CTTGCGCATGTTCTGTTAATCC | This study |
| pmtA REV | TCAAGTTGTGAGTGGTGCTATT | This study |
| 16S rRNA FWD | GTGGAGGGTCATTGGAAACT | This study |
| 16S rRNA REV | CACTGGTGTTCCTCCATATCTC | This study |
| agrC complementation | ||
| agrC FWD BamHI | TGGATCCAGAGGAGAAATTAAATGAT | This study |
| agrC REV SstI | CCCGAGCTCCTAGTTGTTAATAATTTCAAC | This study |
| Transposon insertion sequencing | ||
| RP45 | GAGTGTGATGATAAGTGGGAAGGAC | This study |
| SS1905 | GGTAAACTATGATTCACGACGACTAG | This study |
| Plasmids | ||
| pCL15 | E. coli-S. aureus shuttle vector | 70 |
| pCL15-Pspac-agrC | agrC complementation vector | This study |
| pTXΔpsmα1-4 | psm complementation vector | 39 |
| pTXΔ16 | Empty vector | 81 |