Figure 2.

Lack of bleach-induced transduction activity in Gcaps^−/−Gnat1^−/− rods. A, Current recorded in darkness from a dark-adapted unbleached rod (dark-adapted), a rod 2 h after 1.4% rhodopsin bleach (bleached, 2 h), and from an unbleached rod in 500 nm dim background light producing an estimated 1.4 R* s⁻¹ (dark-adapted +dim background light). B, Averaged power spectra of the dark current recorded from rods that were dark-adapted (dark-adapted, black circles, n = 10) or after a 1.4% rhodopsin bleach and incubation in darkness for 2 h (bleached, 2 h, red squares, n = 10). Dashed line is the power spectrum from unbleached rods under dim background light (n = 3) as in A. C, Difference in the power spectrum between bleached Gcaps^−/− and Gcaps^−/−Gnat1^−/− rods (red circles). Data were fitted with scaled power spectrum of single photon response (red dash line) with a scaling factor of 2.35. Plots in B and C are shown as mean ± SEM.