Figure 5.

Conditional SnoN KO impairs proliferation and promotes cell cycle exit of granule neuron precursors in the postnatal cerebellum. A–C, P13 conditional SnoN KO and control littermate mice were injected with EdU, and 24 h later, the cerebellum was subjected to histochemical analyses using the EdU detection reagent and Ki67 antibody. EdU⁺/Ki67⁺ cells represent granule neuron precursors, which remained in the cell cycle, whereas the EdU⁺/Ki67⁻ cells represent granule neuron precursors that have exited the cell cycle and differentiated into postmitotic granule neurons within 24 h. The number of EdU⁺/Ki67⁺ granule neuron precursors was significantly reduced in the EGL of lobules VI (A), VII (B), and VIII (C) upon conditional KO of SnoN. By contrast, the number of EdU⁺/Ki67⁻ postmitotic granule neurons was significantly increased in lobules VI (A), VII (B), and VIII (C) upon conditional KO of SnoN. Graphical data are presented as mean ± SEM. **p < 0.01 (t test). n = 4 mice.