Figure 5.

Paranodal Caspr dimer immunostaining in CNS tissue is progressively reduced with increasing glycolipid deficiency. A, CST^−/− × GalNAc-T^−/− and CST^−/− × GD3s^−/− mice have significantly fewer Caspr dimers per FOV compared with WT and GalNAc-T^−/− mice and are not significantly different from each other (n = 2–5/genotype). Caspr dimer number is improved to levels within GalNAc-T^−/− mice range, but not WT, in CST^−/− and CST^−/− × GalNAc-T^−/−-Tg(neuronal) mice, which both display significantly more Caspr dimers than CST^−/− × GalNAc-T^−/− mice. Box-and-whisker plots are used to display the spread of all data points collected from each animal and means were calculated per genotype for statistical analysis (one-way ANOVA followed by Tukey's post hoc tests to compare multiple comparisons, indicated on the graphs as follows: *p < 0.05; **p < 0.01; ***p < 0.001). B, Representative images of OpN sections from each genotype double-immunostained for Caspr (red) and the nodal marker AnkyrinG (green) showing the reduction in Caspr dimer number flanking ankyrin G clusters with diminishing glycolipid expression. Scale bar, 10 μm.