Table 1.
Primer sequences used in PCR assays with OLR1 gene fragments
| Primer name | Primer sequence (5′ to 3′) | Annealing temperature (°C) | Product length (bp) | Amplified region |
|---|---|---|---|---|
| L1 | ACTCCAGCAGGAACTCACA AATGATAAGCCAACTTGGT |
56.7 | 336 | Exon 1 and intron 1 |
| L2 | ATATCTGATATTGAATCCCA ATGAGGGCTTATAAACA |
60.0 | 381 | Intron 1, exon 2, and intron 2 |
| L3 | TTATTGGGAATTGGAATTGG CTCTATTTTTGTCATCCT |
57.2 | 448 | Intron 2, exon 3, and intron 2 |
| L4 | GTTAAGAATTGTAGAAATAT GACGCCCCACTTGTAAG |
63.5 | 653 | Intron 3, exon 4, intron 4, and exon 5 |
| L5 | AAGGCGAATCTATTGAGAGC CCTAGAAGAAAGCATAGGAC |
60.4 | 360 | Exon 6 and 3′UTR |
PCR, polymerase chain reaction; OLR1, oxidized low density lipoprotein receptor 1, UTR, untranslated region.