Figure 1.

The amino acid sequence of the constructed HPV16 E6mE7m immunogen. E6mE7m proteins were combined with mutated E6 and E7. The mutated sites were highlighted or marked for inactivating the oncogenic activity of E6 and E7. The alignment of E6mE7m and wild-type E6 and E7 was representative. Additionally, a HisTag sequence was included at the C-terminus of the proteins, and the DNA sequence of E6mE7m was-modified using the codon usage of E. coli. For rlipo-E6mE7m protein expression, the region containing the lipid signal peptide was inserted into the expression plasmid.