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. 2018 Dec 1;8(12):2528–2537.

Figure 3.

Figure 3

rlipo-E6mE7m proteins activate bone marrow-derived dendritic cells (BM-DCs). BM-DCs from wild-type mice were cultured in medium supplemented with LPS (0.1 μg/ml), rE6mE7m (10 μg/ml) or rlipo-E6mE7m (10 μg/ml) in the presence or absence of polymyxin B (20 μg/ml). After a 24-hr incubation, the expression of the dendritic cell surface markers CD40 (A) and CD80 (B) was analyzed using flow cytometry. The experiments were performed in triplicate, and the mean fluorescence intensity (MFI) of the cells cultured in medium alone was defined as 1. (C) TNF-α secretion by dendritic cells was stimulated by rlipo-E6mE7m. BM-DCs were cultured in medium supplemented with LPS (0.1 μg/ml), rE6mE7m (10 μg/ml) or rlipo-E6mE7m (10 μg/ml) in the presence or absence of polymyxin B (20 μg/ml). After a 24-hr incubation, the supernatants were harvested and analyzed for TNF-α production by ELISA. (D) BM-DCs isolated from wild-type and TLR2 knockout (TLR2 KO) mice were cultured in medium supplemented with LPS (0.1 μg/ml), rE6mE7m (10 μg/ml) or rlipo-E6mE7m (10 μg/ml), and then TNF-α production was detected by ELISA after a 24-hr incubation.