Figure 4.

HEF1 regulates migration and invasion and induces Rac1 activation with EMT. A. Western blot analysis on HEF1 expression in 5637 cells after transfected with shHEF1 or pcDNA^TM3.1-HEF1. B. Transwell assays on influences of HEF1 in 5637 and T24 cells after transfection above. C. HEF1 influences on EMT related protein analyzed by Western blot in 5637 and T24 cells. D. Western blot analysis on Rac1 protein after transfected with shHEF1 or pcDNA^TM3.1-HEF1 in 5637 cells. **for P<0.01. All experiments were repeated in triplicate.