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HEF1 knockdown restores the malignant properties induced by SPAG9. A. Transfection combinations of shSPAG9 + pcDNATM3.1-HEF1 or pcDNATM3.1-SPAG9 + shHEF1 and its control vectors were first implemented in 5637 and T24 cells, then protein was extracted and analyzed by Western blot. B. Transwell assays on evaluating the migration and invasion abilities after co-transfection above in 5637 and T24 cells. *for P<0.05, **for P<0.01. All experiments were repeated in triplicate.
