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HEF1 acts as mediator in SPAG9 induced Rac1 signaling pathway. A. Western blot analysis on SPAG9 transfection affecting Rac1 activation in vivo. B. Western blot analysis of SPAG9 transfection influences on Rac1 in vitro in 5637 cells. C. Western blot analysis on HEF1 antagonism after transfection of pcDNATM3.1-HEF1 and pcDNATM3.1-HEF1 on Rac1 activation in 5637 cells. All experiments were repeated in triplicate.
