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. 2018 Dec 1;8(12):2507–2517.

Figure 3.

Figure 3

PAX3 is direct target of miR-485-5p in U251 and U87 cells. A. Predicted binding sites of wild-type (WT) and mutated sequences of miR-485-5p in the 3’-UTR of PAX3 mRNA. B. Luciferase assays of U251 and U87 cells transfected with pGL3-PAX3 3’-UTR WT or pGL3-PAX3 3’-UTR Mut reporter with miR-485-5p mimic. *P < 0.05. C. Western blot analysis of PAX3, JNK and p-JNK protein levels in U251 and U87 cells after transfection with miR-485-5p and miR-NC. D. TCGA database showing PAX3 expression in NBTs and GLIOMAs. E. CGGA database showing PAX3 expression in LGG and HGG. F. Pearson’s correlation analysis of the relative expression levels of miR-485-5p and the relative PAX3 mRNA levels in HGG.