Effects of co-treatment on EMT in PC cells. (A) PANC-1 and (B) MIA PaCa-2 cells were treated with the indicated concentration of CUDC-101 and/or gemcitabine for 48 h. Expression of EMT markers (E-cadherin, ZO-1, Vimentin) were detected by IF staining. (C) Western blot analyses was performed for EMT-associated markers (E-cadherin, Vimentin, MMP-9, Snail and Slug).