Figure 4.

The secreted AREG mediates IBE-induced EGFR kinase activation. A. H292 parental and 1% IBE stable clone cells were seeded in IBE free medium for 24 hours, then conditioned medium was collected and used for culture of H292 parental cells for 30 minutes. B. Total lysates extracted from cells cultured with conditioned medium were performed with western blot analysis by with indicated antibodies. C. The expression of EGF, TGF, HB-EGF and AREG mRNA in H292 cells and their 1% IBE clone were analyzed by real-rime PCR. D. The levels of AREG in the culture medium of H292 parental cells and their 1% and 5% IBE stable clones were measured in the ELISA assays. Results were expressed as mean ± S.E.M. of two independent experiments. *: P < 0.05; **: P < 0.01 as compared with control group.