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. 2018 Dec 1;8(12):2494–2506.

Figure 2.

Figure 2

LINC00163 overexpression suppresses proliferation, migration and invasion in lung cancer. A. LINC00163 was overexpressed in A549 and H1299 through transfection with pcDNA3-LINC00163. And the efficiency of LINC00163 overexpression was analyzed by qRT-PCR. B and C. A549 and H1299 cells were transfected with pcDNA3-LINC00163. And CCK8 assay was used to clarify cell proliferation at different time points including 1 day, 2 days and 3 days. oeLINC00163 indicates overexpression of LINC00163. D. A549 and H1299 cells were transfected with pcDNA3-LINC00163. Colony formation assay was used to analyze cell proliferation. E and F. After transfection, images and quantification of cell migration and invasion of A549 and H1299 were determined by transwell assay. G. LINC00163 was knocked down in A549 and H1299 cells by transfection with si-LINC00163. The efficiency of knockdown was analyzed by qRT-PCR. siRNA-NC: siRNA negative control. H and I. After infection, CCK8 assay was used to analyze cell proliferation in A549 and H1299 cells. J. Colony formation assay indicated that LINC00163 silencing increased colony number. K and L. Cell migration and invasion was determined by Transwell assay after LINC00163 silencing. *P<0.05 and **P<0.01.