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. 2018 Dec 1;8(12):2494–2506.

Figure 5.

Figure 5

LINC00163 recruits ARID1A to initiate TCF21 expression. A. The subcellular localization of LINC00163 in cytoplasm and nuclear was analyzed by qRT-PCR. B. The subcellular localization of LINC00163 in LINC00163-overexpressing A549 and H1299 cells was analyzed by qRT-PCR. C. Chromatin isolation by RNA purification (ChIRP) assay using LINC00163 biotin probes showed that LINC00163 was enriched on the promoter of TCF21 in A549 cells. The biotin-labeled probes against LINC00163 were incubated with the ultrasonically treated cell lysates. Then the precipitated DNA fragment was isolated and analyzed by qPCR. D. RIP assay showed that LINC00163 interacts with ARID1A. E. The interaction between LINC00163and ARID1A in lung cancer was analyzed by RNA pulldown assay. A549 cell lysates were used. F. ChIP assay showed that ARID1A was enriched on the promoter of TCF21. G. ChIP assay showed that LINC00163 silencing inhibited the enrichment of ARID1A on TCF21 promoter. H. qRT-PCR analysis for TCF21 expression in described cells. **P<0.01.