Figure 4.

Suppression of malignant behaviors in osteosarcoma cells by knockdown of YEATS4 and the knockdown effect according to TP53 mutation status. A. Effects of YEATS4 knockdown by siRNA (siRNA-YEATS4) compared with those of the control siRNA in U2OS (wild-type TP53) and SaOS2 (null TP53) cell lines. B. Effects of knocking down endogenous YEATS4 on cell proliferation at the indicated times. The results are the means ± SD (bars) for quadruplicate experiments. The Mann-Whitney U-test was used for the statistical analysis: *P < 0.05. Representative results of the population in each phase of the cell cycle in osteosarcoma cells assessed by FACS at 72 h after treatment with siRNA. Representative results of the apoptosis assay in GC cells at 72 h after treatment with siRNA. C. Western blotting analyses showed that knockdown of YEATS4 by transfection with siRNA-YEATS4 induced the production of p21 and suppressed the Notch2 expression in U2OS and SaOS2 cells. In U2OS (wild-type TP53) cells only, knockdown of YEATS4 also induced phosphorylation activation of AKT.