Figure 5.

Molecular mechanisms by which overexpression of YEATS4 contributes to malignant potential in GC cells. A. Knockdown of YEATS4 by transfection with siRNA-YEATS4 induced the production of p21 and suppressed Notch2 expression. In NUGC4 (wild-type TP53) cells, knockdown of YEATS4 also induced phosphorylation activation of AKT, the cleavage of caspase 3 and PARP. B. Knockdown of YEATS4 suppressed Notch2 expression in both NUGC4 (wild-type TP53) and HGC27 (mutant-type TP53) GC cells. Moreover, the distribution of YEATS4 in GC cells was similar to that of Notch2. C. A hypothetical model of the overexpression/activation of YEATS4 in GC cells.