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. 2018 Dec 1;8(12):2387–2401.

Figure 5.

Figure 5

Identification of PDE4D as a miR-203a-3p target. A. Quantitative RT-PCR analysis of PDE4D expression levels in the same 8 pairs of CRC and normal tissue samples and in the same CRC cell lines. B. Over-expression of miR-203a-3p significantly suppressed PDE4D at both the mRNA and protein levels in SW480 and SW1116 cells. C. Silence of endogenous miR-203a-3p significantly increased PDE4D at both the mRNA and protein levels in HCT116 and SW1116 cells. D. Binding sites of miR-203a-3p at the 3’-UTR of PDE4D mRNA. E. PDE4D is a target gene of miR-203a-3p in HCT116 and SW480 cells based on the luciferase reporter assay. Each assay was repeated three times. The data represent means ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.