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. 2018 Dec 1;8(12):2564–2574.

Figure 4.

Figure 4

miR-338-3p suppresses RAB23 expression by directly targeting the 3’-UTR of RAB23 mRNA. A. Sequence alignment of miR-338-3p and its predicted binding sites in RAB23 3’-UTR. B. Relative expression of RAB23 mRNA was measured by qRT-PCR in PC-3 and DU145 cells infected with the miR-338-3p-overexpressing lentivirus or control lentivirus. C. Western blot analysis of RAB23 expression in the indicated cells. D. IHC staining against RAB23 in the xenograft tumor tissues. E. A luciferase reporter assay. A vector containing Wt RAB23 3’-UTR or Mt RAB23 3’-UTR was cotransfected into prostate cancer cells together with the indicated oligonucleotides. A luciferase activity ratio is presented as firefly luciferase activity/Renilla luciferase activity. F. Relative expression of RAB23 mRNA in 24 paired samples. G. Pearson’s analysis of correlation between miR-338-3p and RAB23 mRNA levels in human prostate cancer tissue samples (r = -0.532, P = 0.007). H. RAB23 protein expression in prostate cancer tissue samples was analyzed by IHC (**P < 0.01).