Figure 3.

Serum leptin level was consistent with the tooth movement change during the experiment course and the effects of leptin on osteoclastogenesis in RAW264.7 cells. A. Serum leptin level was significantly elevated before and after 7 days of force application. No significant difference was noted on day 3 and day 5. B. Immunofluorescence staining confirmed that leptin receptor was expressed on RAW264.7 cells. C. TRAP staining showed that administration of leptin (100 ng/ml) inhibited osteoclatogenesis in RAW264.7 cells. D. Relative RNA expression of osteoclast-specific functional gene was determined by quantitative reverse-transcription polymerase chain reaction (q-PCR). β-actin served as a loading control; **P<0.01, *P<0.05; NS, not statistically significant.