Figure 4.

Effects of leptin on force induced orthodontic tooth movement in mice. A. Schedule of the experiment. Orthodontic force was applied to mice. Mice were sacrificed at 3 d, 5 d and 7 d after force application. Intraperitoneal injection of leptin or vehicle was performed every other day since one day prior to force application. B. Trends of decreased body weight was noted, while no statistically significant difference was found. C. Representative intra-oral photograph of tooth movement mice with injection of leptin or vehicle at 3 day, 5 day and 7 day of force application. D. MicroCT representative image of tooth movement in mice with injection of leptin or vehicle after 7 days of force application. E. Amount of orthodontic tooth movement on 3 day, 5 day and 7 day after force application in mice with leptin or vehicle administration. The blue arrows indicated tooth movement distances, M1: first molar. M2: second molar. n=5 in each group, error bars, means ± SD; scale bar: 400 μm; **P<0.01, *P<0.05; NS: not statistically significant.