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. 2018 Dec 15;10(12):4280–4289.

Figure 1.

Figure 1

BMSCs and EPCs proliferations in the co-culturing system. A, B: The proliferative abilities of BMSCs and EPCs were detected by CCK-8 assay, the experiments were divided into five groups including BMSCs alone, EPCs alone, co-culturing BMSCs with EPCs at the ratio of 1:1, 1:2 and 2:1 respectively. The OD values were used to quantify cell proliferation. C, D: Western blot was performed to detect cell cycle associated proteins including cyclin D1, cyclin E2, CDK2, CDK4 and CDK6, the intensity of the protein bands were employed to evaluate protein levels. E-H: Image J software was used to quantify proteins according to the gray values. (*, P<0.05 VS B(alone) or E(alone) control groups).