Figure 2.

Cell apoptosis was affected by co-culturing BMSCs with EPCs at the ratio of 1:1, 1:2 and 2:1 respectively. A, B: Western Blot was employed to investigate apoptosis associated proteins including Bcl-2, Bax and Caspase3 in BMSCs and EPCs after culturing the two cells alone or co-culturing the BMSCs with EPCs at the ratios of 1:1, 1:2 or 2:1 respectively for 4 hours, the intensity of the protein bands were employed to evaluate protein levels. C, D: Image J software was used to analyze the grey values of the protein bands, the proteins were normalized by β-actin. E: Flow cytometry (FCM) was performed to detect cell apoptosis rates in the BMSCs or EPCs alone groups and co-culturing the two cells at the ratio of 2:1 for 4 hours, BMSCs and EPCs were separated and stained with Annexin V-FITC and PI. (*, P<0.05 VS B(alone) or E(alone) control groups).