Figure 3.

ISL downregulated expressions of IL-6 and HMGB-1 both in vivo and in vitro. A and B. The levels of mRNA of IL-6 were determined by quantitative Real-Time PCR. LPS increased IL-6 significantly while ISL downregulated the levels of IL-6. The samples were respectively isolated from cells and mouse. C. Immunohistochemistry was performed to detect HMGB-1 in mouse. LPS activated HMGB-1 expression in glomerulus and kidney tubules. ISL reduced the expression of HMGB-1 especially in renal tubular epithelial cells. (magnification, ×400). D, E. Total protein of cells was tested for HMGB-1 by western blot. Quantification of western blotting gel images was presented in graphical form. ISL with two concentrations in cells both can decrease expression of HMGB-1. F, G. Total kidney tissue protein of mouse was tested for HMGB1 by western blot. ISL reduced HMGB-1 expression after LPS-induced overexpression. **P < 0.01 vs. the LPS group, ***P < 0.001 vs. the LPS group, ###P < 0.001 vs. the Ctrl group.