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. 2018 Dec 15;10(12):3969–3977.

Figure 5.

Figure 5

MiR-4262 functionally targets GALNT4 in colon cancer cells. To further investigate whether GALNT4 may be a target gene of miR-4262, the luciferase reporter plasmids containing either wild-type or mutant 3’-UTRs of GALNT4 were constructed. The luciferase reporter assay was set up to identify the direct miR4262-GALNT4 interaction. The relative luciferase activity was significantly lower in cells after 48 hours co-transfection with miR-4262-modified plasmids and p3’-UTR-GALNT4 or miR-4262. There was a statistically difference between cells co-transfected with p3’-UTR-GALNT4-mut and cells co-transfected with the pre-miR-4262. Those consequences indicated that miR-4262 could specifically bind to seed zone of GALNT4 3’-UTR to inhibit its expression, while mut vector could not combined with miR-4262 to decrease the relative luciferase activity. *P<0.05 compared to miR-scramble. NS: non-significant. N=5.