Figure 4.

Muscone exerted anti-inflammatory effects by inhibiting NF-κB and NLRP3 inflammasome activation both in vivo and in vitro. A. After being incubated for 24 h, the protein level of TLR4 in BMDMs was measured by western blot. B. The protein level of TLR4 in mouse hearts at the 4^th week after MI was measured by western blot. C. Western blot analysis of p-p65, p65, p-IκB-a, IκB-a, NLRP3, pro-caspase1and caspase1 in BMDMs after being incubated for 24 h. β-actin was used as a loading control. D. Western blot analysis of p-p65 and NLRP3 in mouse hearts at the 4^th week after MI. GAPDH was used as a loading control. E. Representative photomicrographs of immunohistochemical staining for p-p65 and NLRP3 in paraffin embedded heart sections at the 4^th week after MI (bar = 50 μm). All results are representative of three independent experiments and shown as mean ± SD. **P < 0.01, ***P < 0.001 versus control or sham group, #P < 0.05, ##P < 0.01versus LPS or MI group.