Figure 2.

Hiptopathological changes of livers in mice. Liver tissues were harvested 12 h after Con A administration intravenously. Liver samples were fxed in 4% buffered paraformaldehyde for at least 24 h. Sections (4-5 μm) on slides were deparafnized in xylene, rehydrated in decreasing concentrations of ethanol, and stained with hematoxylin and eosin (H&E). All sections were graded blindly by three pathologists under light microscopy according to the following criteria: 0, none; 1, individual cell necrosis; 2, ≤30% lobular necrosis; 3, ≤60% lobular necrosis; 4, >60% lobular necrosis. A-C represented the microscopic appearance of liver tissue in control, Con A and anti-CD200 group. D represented the severity of liver injury scored by three blinded Pathologists. *P<0.01 compared to control group, #P<0.01 compared to Con A group.