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Coexpression of BDNF with the neuropeptide CGRP or the cold receptor, TRPM8. A, Double in situ hybridization confirmed that some BDNF mRNA-positive neurons (green in A, D), coexpress CGRP (red in B, C). D–F, Double in situ hybridization for GFP (red in E, F) and BDNF (green in D) in DRG neurons from TRPM8-GFP knock-in mice show that ∼23% of BDNF-positive neurons coexpress TRPM8. Scale bar = 100 μm.
