Figure 4.

BDNF expression in sensory ganglia and spinal cord neurons of the adult mouse. A, B, mRNA expression of BDNF in adult DRG (A) and spinal cord (B) neurons revealed by in situ hybridization. BDNF mRNA was detected in all laminae of the dorsal horn. C–F, In the adult BDNF-LacZ knock-in reporter mice, β-Gal expression was also found in spinal cord (C) and trigeminal nucleus caudalis neurons (D). We only recorded β-Gal expression in neurons (NeuN+, green in E), not in microglia (Iba1+, green in F). Scale bars = 100 μm (A, B, D–F) and 25 μm (C). DC: dorsal column; I, II, III–IV: laminae I, II and III–IV.