Figure 6.

Baseline nociceptive responsiveness of the BDNF-cKO mice A, B, BDNF cKO mice (Tam; gray) displayed normal behavior compared to vehicle-injected controls (Ctrl) on the rotarod test (A) of motor behavior and Von Frey test (B) of mechanical thresholds. C, Tamoxifen-treated cKO mice also responded normally on the hotplate test at all tested temperatures. D, Male knock-out mice showed a significant hyposensitivity in the tail immersion test compared to all other groups. As all knock-out mice responded normally in the Hargreaves (E), capsaicin (F), and acetone (G) tests results from male and female were combined. Numbers of animals are indicated on graphs. Data are mean ± SEM. For A, B, E–G, statistical significance was determined by Student’s t test. For C, statistical significance was determined by ANOVA with Bonferroni correction for multiple comparisons. For D, statistical significance was determined by two-way ANOVA with Bonferroni correction for multiple comparisons; *p < 0.05, **p < 0.01.