Figure 11.

OFQ inhibits the GnRH neuron firing rate in acute brain slices, independent of GABAergic and glutamatergic inputs. A, Left, Electrophysiological recording of adult GFP-tagged GnRH neurons showing that OFQ (1 µm, 100 nm, and 10 nm) evoked a potent decrease in GnRH neuron firing rate. Right, Summary data showing quantification of firing rate (in Hz) in individual GnRH neurons tested at the different concentrations during the control (CTRL), treatment (OFQ), and washout (WO) periods. The values represent the average of 1 s bins for the last 1 min of each period identified on the traces. B, Left, OFQ (10 nm) in the presence of AABs (BIC, 20 µm; CNQX, 10 µm; d-AP5, 20 µm) still decreased GnRH neuron firing rate. Right, Summary data showing quantification of the firing rate (in Hz) in individual GnRH neurons tested during the control (CTRL), pretreatment (AAB), treatment (+OFQ), and washout (WO) periods. The values represent the average of 1 s bins for the last 1 min of each period identified on the traces.