Table 3:
Inhibition of GnRH neurons
| Paradigms with OFQ(1-13) | Period (with OFQ) − Period (without OFQ)/Period (with OFQ) (change in %) | Cells (n) |
Explants (N) |
|
|---|---|---|---|---|
| a | SFM – OFQ | −75.6 ± 2.0 | 144 | 5 |
| b | UFP – UFP + OFQ | −17.3 ± 3.3* (p < 0.0001) | 112 | 3 |
| c | SB – SB + OFQ | −29.5 ± 2.5* (p < 0.0001) | 244 | 6 |
| d | PTX pretreated: SFM – OFQ |
−17.4 ± 4.4* (p < 0.0001) | 88 | 3 |
| e | IBMX + FSK – IBMX + FSK + OFQ | −68.2 ± 2.6 (p > 0.9864) | 161 | 3 |
| f | Cs – Cs + OFQ | −28.6 ± 3.6* (p < 0.0001) | 168 | 5 |
| g | Ba – Ba + OFQ | −12.0 ± 4.9* (p < 0.0001) | 36 | 4 |
| h | TPNQ – TPNQ + OFQ | −72.9 ± 2.5 (p > 0.9999) | 80 | 3 |
| i | PMA – PMA + OFQ | 18.7 ± 16.8* (p < 0.0001) | 113 | 4 |
| j | SFM – BAC | −86.2 ± 1.9 | 88 | 4 |
| k | TPNQ – TPNQ + BAC | −93.5 ± 1.5 (p = 0.9206) | 53 | 3 |
| l | PMA – PMA + BAC | 19.9 ± 20.5* (p < 0.0001) | 92 | 3 |
Concentrations used: OFQ, 100 pm; UFP, 10 nm; SB, 10 nm; PTX, 250 ng/ml; IBMX, 10 µm; FSK, 1 µm; Cs, 5 mm; Ba, 400 µm; TPNQ, 500 nm; PMA, 50 nm; BAC, 10 µm. Data are expressed as the mean ± SEM.
*
Significant difference (p < 0.01), using one-way ANOVA, followed by Dunnett’s multiple-comparisons test using OFQ (row a) as a reference for rows b–i or BAC (row j) as a reference for rows k and l).