Table 2.
: Expression of additional clock (Per1, Clock) and inflammatory (Iba1, Mhc II) genes at 2 d postsurgery in the lesion epicenter and lumbar spinal cord (data only for genes not shown in figures)
| Female | Male | Significant differences | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ZT6 | ZT18 | ZT6 | ZT18 | |||||||
| Gene | Sham | SCI | Sham | SCI | Sham | SCI | Sham | SCI | Main effect | Interactions |
| Per1 | ||||||||||
| Lesion Lumbar |
1.00 ± 0.10 1.00 ± 0.05 |
0.39 ± 0.06 1.04 ± 0.29 |
1.19 ± 0.07 0.97 ± 0.09 |
0.23 ± 0.03 0.70 ± 0.03 |
1.01 ± 0.17 0.78 ± 0.08 |
0.35 ± 0.06 0.57 ± 0.07 |
2.01 ± 0.57 0.84 ± 0.12 |
0.34 ± 0.01 0.50 ± 0.02 |
SCI: ZT6 + 18 SCI, sex |
Sham × ZT N.S. |
| Clock | ||||||||||
| Lesion Lumbar |
1.00 ± 0.16 1.00 ± 0.03 |
0.32 ± 0.03 0.71 ± 0.10 |
1.21 ± 0.18 1.07 ± 0.06 |
0.27 ± 0.04 0.64 ± 0.04 |
0.90 ± 0.18 0.94 ± 0.07 |
0.36 ± 0.09 0.53 ± 0.07 |
1.56 ± 0.33 0.83 ± 0.08 |
0.29 ± 0.03 0.45 ± 0.04 |
SCI SCI |
Sham × ZT Sham × ZT |
| Iba1 | ||||||||||
| Lesion Lumbar |
1.00 ± 0.21 1.00 ± 0.07 |
0.75 ± 0.03 1.36 ± 0.04 |
0.86 ± 0.08 0.96 ± 0.08 |
0.93 ± 0.09 1.38 ± 0.13 |
0.69 ± 0.09 1.32 ± 0.05 |
0.41 ± 0.07 2.06 ± 0.45 |
0.59 ± 0.04 1.23 ± 0.12 |
0.78 ± 0.14 1.54 ± 0.18 |
Sex N.S. |
SCI × ZT;ZT6 × inj. N.S. |
| Mhc II | ||||||||||
| Lesion Lumbar |
1.00 ± 0.17 1.00 ± 0.19 |
0.21 ± 0.05 0.50 ± 0.05 |
0.82 ± 0.09 0.54 ± 0.21 |
0.21 ± 0.03 0.54 ± 0.18 |
0.65 ± 0.14 1.59 ± 0.38 |
0.23 ± 0.05 0.86 ± 0.14 |
0.79 ± 0.14 1.09 ± 0.30 |
0.22 ± 0.05 0.54 ± 0.11 |
SCI SCI, sex |
N.S. N.S. |
For statistical comparisons (far right column): SCI, significant main effect of SCI vs sham; ZT, significant main effect of time (ZT6 vs ZT18); sex, significant main effect of sex; Sham × ZT, significant interaction between sham and ZT; N.S., no significant difference.