Table 1.

Ion concentrations of CS extracts diluted with DMEM medium and PBS

	Ca [µg mL−1]	P [µg mL−1]	Si [µg mL−1]
DMEM	59.33 ± 0.97	25.22 ± 0.34	0.02 ± 0.01
(1)CS in DMEM	5.11 ± 0.25b)	6.37 ± 0.09b)	113.59 ± 0.30b)
CS/1/16 in(1)	56.19 ± 0.46a)	23.92 ± 0.38a)	6.66 ± 0.05b)
CS/1/32 in(1)	57.89 ± 0.62	24.98 ± 0.68	3.36 ± 0.04b)
CS/1/64 in(1)	58.92 ± 0.73	25.24 ± 0.70	1.63 ± 0.02b)
CS/1/128 in(1)	59.47 ± 0.42	25.01 ± 0.28	0.80 ± 0.02b)
CS/1/256 in(1)	59.62 ± 0.55	25.13 ± 0.52	0.41 ± 0.01b)
PBS	0.06 ± 0.00	274.44 ± 1.00	0.12 ± 0.01
(2)CS in PBS	0.05 ± 0.01	32.52 ± 0.44d)	283.88 ± 3.43d)
CS/1/16 in(2)	0.08 ± 0.01	260.22 ± 0.55d)	17.42 ± 0.37d)
CS/1/32 in(2)	0.06 ± 0.00	268.08 ± 0.63c)	9.08 ± 0.75d)
CS/1/64 in(2)	0.06 ± 0.00	270.91 ± 0.81	4.53 ± 0.18d)
CS/1/128 in(2)	0.05 ± 0.00	273.06 ± 0.10	2.26 ± 0.03d)
CS/1/256 in(2)	0.05 ± 0.00	273.23 ± 0.79	1.25 ± 0.02d)

^a) p < 0.05 and

^b) p < 0.001 when compared with concentration of ions in DMEM medium.

^c) p < 0.05 and

^d) p < 0.001 when compared with concentration of ions in PBS; All data are obtained from three independent experiments; mean ± SD.