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. 2018 Oct 25;6(1):1801039. doi: 10.1002/advs.201801039

Figure 2.

Figure 2

Engineering CAR‐T cells using CRISPR‐Cas9 for precision cancer immunotherapy. a) The CD19 CAR was precisely inserted into the TRAC locus by CRISPR‐Cas9‐mediated genome editing. The insertion also caused the knockout of T cell receptors, delaying T cell exhaustion. b) Quantification of T cell exhaustion markers expression on the engineered T cell. Insertion of CAR into the T cell receptors locus (TRAC‐1928z) significantly reduced the expression of T cell exhaustion markers, showing huge advantage over non‐targeted construction approaches. c) The 2‐in‐1 engineering strategy generated CAR‐T cells with more robust anticancer efficacy, increasing the survival of treated mice. Reproduced with permission.48 Copyright 2017, Springer Nature.