Table 5.
Gross morphologic observations in zebrafish larvae identify known toxicants including hepatotoxic amiodarone
| Compound/Dose Range | Gross Morphology | Concentration, μM | ||||||
|---|---|---|---|---|---|---|---|---|
| Dose range | 200 | 66 | 20 | 6.6 | 2 | 0.66 | DMSO | |
| Menadione | Live/deada | 0/4 | 0/4 | 0/4 | 4/0 | 4/0 | 4/0 | 4/0 |
| Visual toxicityb | 1/4 | 2/4 | 0/4 | 0/4 | ||||
| Amiodarone | Live/dead | 0/4 | 0/4 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 |
| Visual toxicity | 2/4 | 3/4 | 0/4 | 1/4 | 0/4 | |||
| Dose range | 1000 | 300 | 100 | 30 | 10 | 3 | DMSO | |
| Caffeine | Live/dead | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 |
| Visual toxicity | 3/4 | 4/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | |
| Dose range | 20 | 6.6 | 2 | 0.66 | 0.2 | 0.07 | DMSO | |
| CCCP | Live/dead | 1/4 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 |
| Visual toxicity | 0/1 | 2/4 | 3/4 | 3/4 | 4/4 | 2/4 | 0/4 | |
| Rotenone | Live/dead | 1/4 | 3/4 | 4/0 | 4/0 | 4/0 | 4/0 | 4/0 |
| Visual toxicity | 1/1 | 0/3 | 1/4 | 2/4 | 3/4 | 2/4 | 0/4 | |
Abbreviations: CCCP, Carbonyl cyandide m-chorophenyl hydrazone; DMSO, dimethyl sulfoxide.
a
Translucent (live), Opaque (dead).
b
Bent tail, distended peritoneum, edema, pericardial congestion.