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. 2019 Jan 8;19:9. doi: 10.1186/s12935-018-0714-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The expression of wild-type CD59 is beneficial to the survival of Jurkat cells. a The cell vitality detected by CCK8 assay. b The dead cells detected by trypan blue staining. c Quantitative analysis of blue-stained cells. d Lytic cells detected by Dye release assay. e The apoptosis detected by flow cytometry. f Quantitative analysis of apoptosis. g The protein expression of Bax and Bcl-2 detected by western blot. h Quantitative analysis of mRNA expression of Bax and Bcl-2. i Quantitative analysis of protein expression of Bax and Bcl-2. j The content of IL-2 in culture medium detected by ELISA. All experiments were performed three times. *P < 0.05. n.s no significant