Table 1. HP1a-deficient ovaries exhibit various nuclear defects.
Genotype | Multiple nuclei | Overcondensed nuclei | Joined egg chambers | Accumulation of early stage egg chambers | n |
---|---|---|---|---|---|
w1118 | 0 | 6 (0.0%) | 0 | 0 | 344 |
Su(var)2-504 | 4 (0.0%) | 6 (0.0%) | 0 | 0 | 484 |
Su(var)2-504; HP1a RNAi#1 | 248 (74.6%) | 194 (58.4%) | 92 (27.7%) | 68 (20.4%) | 332 |
Su(var)2-504; EGFP RNAi#1 | 8 (0.0%) | 10 (0.0%) | 0 | 2 (0.0%) | 396 |
Su(var)2-504; HP1a RNAi#2 | 280 (85.3%) | 172 (52.4%) | 180 (34.1%) | 46 (14.0%) | 528 |
Su(var)2-504; EGFP RNAi#2 | 16 (0.0%) | 4 (0.0%) | 4 (0.0%) | 0 | 412 |
For each genotype, the percentage of ovarioles with the indicated phenotypes was calculated from the total number (n) counted in two independent experiments. “Multiple nuclei” indicates > 16 nuclei in an egg chamber (refer to Figure 1C, e and f). The percentage reported in the Results section is the average percentage from those of HP1a RNAi #1 and HP1a RNAi #2. RNAi, RNA interference; EGFP, enhanced GFP.