Table 3. Disrupted nuclear organization in embryos from HP1a-deficient mothers.
| HP1a | H3K9me3 | Lamin | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Maternal genotype | Scattered puncta | Diffuse, not associated with nuclei | Absent | n | Not correlated with HP1a expression | Irregular | Enlarged | Absent (+PH3) | Absent (−PH3) | n |
| w1118 | 3 (0.0%) | 2 (0.0%) | 0 | 382 | 0 | 6 (0.0%) | 0 | 12 (0.0%) | 3 (0.0%) | 500 |
| Su(var)2-504 | 10 (0.0%) | 0 | 0 | 465 | 0 | 4 (0.0%) | 1 (0.0%) | 8 (0.0%) | 0 | 456 |
| Su(var)2-504; HP1a RNAi#1 | 500 (77.8%) | 482 (75.1%) | 472 (73.5%) | 642 | 0 | 552 (80.2%) | 186 (27.0%) | 214 (31.1%) | 15 (0.0%) | 688 |
| Su(var)2-504; EGFP RNAi#1 | 4 (0.0%) | 2 (0.0%) | 0 | 500 | 0 | 5 (0.0%) | 0 | 15 (0.0%) | 218 (47.8%) | 556 |
| Su(var)2-504; HP1a RNAi#2 | 352 (76.9%) | 360 (78.6%) | 360 (78.6%) | 458 | 0 | 431 (76.4%) | 141 (25.0%) | 225 (39.9%) | 20 (0.0%) | 564 |
| Su(var)2-504; EGFP RNAi#2 | 16 (0.0%) | 1 (0.0%) | 0 | 520 | 0 | 5 (0.0%) | 0 | 16 (0.0%) | 200 (44.9%) | 445 |
For each maternal genotype, the percentage of embryos with the indicated phenotypes was calculated from the total number (n) counted in two independent experiments. “Absent” under HP1a expression indicates no HP1a expression on a nucleus. The total number counted (n) for HP1a and H3K9me3 was the same (counted from costained preparations) and are presented in a common column between the two. Percentage reported in the Results section is the average percentage from those of HP1a RNAi #1 and HP1a RNAi #2. “Lamin, Absent (+PH3)” denotes absence of lamin expression in a mitotic (PH3-expressing) cell. “Lamin, Absent (-PH3)” denotes absence of lamin expression in a non-mitotic (not expressing PH3) cell. RNAi, RNA interference; EGFP, enhanced GFP.