Figure 6.

Effects of benomyl on growth and helicase centromere binding in the presence and absence of Pif1 family helicases. (A) Strains of the indicated genotype were spotted in fivefold serial dilutions on YEPD plates containing no or 10 μg/ml benomyl. (B–F) The indicated strains were treated with or without benomyl, and analyzed by ChIP-qPCR. Each strain was tested in triplicate by ChIP-qPCR using three biological replicates. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and *** P ≤ 0.0001. (B) CEN3, (C) CEN12, (D) CEN13, (E) tRNA^ala, and (F) tRNA^tyr. ChIP, chromatin immunoprecipitation; qPCR, quantitative PCR; WT, wild-type; YEPD, YEP and dextrose.