Table 1.

List of primers and probe for the lateral-flow strip and real-time RPA assay based on the HAdVs strain accession no. KX289874

Assay format	Oligo name	Sequence 5′-3’	Genome position
LFD-RPA	ADV-RF-Bio	Bio-GCTCATTGGAACAACTGCCGTAAATAGTGT	20,761–20,790
	ADV-RR	GGCCGAGAACGGTGTACGCAGGTAGACTGTC	21,055–21,025
	ADV-RP-nfo	FITC-CTCGATGACGCCGCGGTGTGGCTGGTGCAC[THF]CTGACCACGTCGAAGA-SpC3	20,979–21,025
Real-time RPA	ADV-RF	GCTCATTGGAACAACTGCCGTAAATAGTGT	20,761–20,790
	ADV-RR	GGCCGAGAACGGTGTACGCAGGTAGACTGTC	21,055–21,025
	ADV-RP-exo	CAGGTAGACTGTCTCGATGACGCCGCGGTGT[FAM]G[THF]CT[BHQ]GGTGCACGCTGACC-SpC3	20,989–21,037

Bio Biotin, FITC fluorescein isothiocyanate, THF tetrahydrofuran, SpC3 C3 spacer, FAM 6-Carboxyfluorescein, BHQ black hole quencher