Table 2.

Sensitivity testing of the real-time and LFD-RPA with a dilution series of plasmid DNA in comparison with real-time PCR results

Template copy number	Real-time PCR	Real-time RPA		LFD-RPA
	Ct Value	Pos. reactions/No. of runs	Means Tt value (min)	Pos. reactions/No. of runs
50,000	20.2	5/5	3.2	5/5
5000	24.1	5/5	5.2	5/5
500	27.7	5/5	5.4	5/5
50	30.8	5/5	5.5	5/5
5	36.1	2/5	6.2	5/5
Neg. control	N.D.	0/5	N.D.	0/5

Ct cycle threshold, Tt time threshold, Neg. Negative, Pos. Positive, N.D. None determined