Table 3.
Bacterial strains, plasmids, and primers used in the current study
| Material | Description | Reference or source |
|---|---|---|
| Bacteria | ||
| L. casei | Plasmid free | ATCC393 |
| E. coli DH5α | Transformation host | CCTCC AB 2012883 |
| L. casei/pPG6121.-BLF | Secreted BLF producer | This work |
| L. casei/pPG6121 | Transformation host | This work |
| Plasmid | ||
| pUC57 | Ampr, ori | Sangon (China) |
| pPG612.1 | Ampr, Cmr, ori | Li et al. |
| pPG612.1-BLF | Ampr, Cmr, ori | This work |
| Primers | ||
| BamHI-F | TGGATCTTGCTTTGAAGGGT | This work |
| BamHI-R | TTTCGTCCCATAGATCTCTGC | This work |
| XhoI-F | ACACGTGAAACAGGTGCTGCT | This work |
| XhoI-R | TTCGTCCAACCAAACCGACT | This work |
ATCC American Type Culture Collection, CCTCC China Center for Type Culture Collection