Fig. 5.

Expression profile of DmFKBP12 protein at late gastrulation stage of the Drosophila embryo. a–d and i H&E staining of embryo at late gastrulation stage. A number of different morphogenic movements has been completed. e–h and j–l display the distribution of DmFKBP12 protein at late gastrulation stage. DmFKBP12 was detected in the differentiated organs which involved in digestive, nervous and muscular system, such as mouth hook, midgut, middle midgut, midgut caecum. Orientation of the embryo is anterior to the top and posterior to the bottom. i–l present advanced late gastrulae than that in e–h. The qualifications of DmFKBP12 distribution in early gastrulae are presented in m. Scale bar for a, e and i is 80 µm, for b–d, f–h and j–l is 40 µm. MH, mouth hook; SLG, salivary gland; GNSO, suboesophageal ganglion; BR, brain, supraesophageal ganglion; AMG, anterior midgut rudipighian; PMUS, pharyngeal musculature; PV, proventriculus; MG, midgut; VNS, ventral nervous system; NF, nerve fibers and tracts; MUS, muscle; YK, yolk; ST, stomodaeum; PMG, Posterior midgut rudiment; MGC, midgut caecum; MMG, middle midgut; TR, trachea. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ***p < 0.0001