Fig. 8.

Fermentation experiments. a Growth curves in EM− and EM+ media. b γ-PGA concentration profiles in EM−. c Electrophoretic separation of γ-PGA collected in the experiments with EM−. d γ-PGA concentration profiles in EM−. The reference strain (PB5383) and the two mutants (PB5716 and PB5716) are indicated as A, B and C, respectively. In a, b, d circles represent average data points, error bars represent the 95% confidence intervals of the mean. Solid and dashed lines indicate experiments in EM− and EM+, respectively. In c the size distribution of the polymer can be qualitatively visualized. The intensity of the signal is comparable only among samples in which the polymer shows a similar distribution