Figure 3.

Casp1^−/− dysbiosis leads to systemic inflammation. A, Plasma cytokines at time of sacrifice. n=10 per group. B, White blood cell (WBC) count and immune subsets during week 5 of cohousing. Ldlr^−/−(Ldlr^−/−) mice, n=18; Ldlr^−/−(Casp1^−/−) mice, n=17. C–D, Female Ldlr^−/− mice aged 12 wk were exposed to fecal microbiota derived from Casp1^−/− or Ldlr^−/− mice for 13 wk while fed high-fat cholesterol (HFC) diet. C, Representative Ly6G-stained slides of the aortic root. Scale bars, 100 μm. D, Number of infiltrated neutrophils per 100.000 μm² characterized by Ly6G-stained slides of the aortic root. Ldlr^−/−(Ldlr^−/−) mice, n=13; Ldlr^−/−(Casp1^−/−) mice, n=12. Data represent mean±SEM. *P<0.05 as determined by unpaired 1-tailed Student t test. IFN indicates interferon; and IL, interleukin.