Fig. 3.

I942 inhibits IL6-promoted JAK/STAT3 signalling in HUVECs.

In the upper panel HUVECs were pre-incubated with 100 μM I942 for 30 min and then incubated with IL6 (5 ng/ml) plus sIL6Rα (25 ng/ml) for different periods of time up to 48 h. Cell extracts were then prepared and immunoblotted with antibodies to phosphorylated and non-phosphorylated STAT3. Densitometric values from 3 separate immunoblots are shown in the lower panel with significant decreases in STAT3 phosphorylation being indicated, ^###, p < 0.001, relative to IL6-stimulated cells.