Fig. 4.

circAGFG1 increases TNBC cell migration and invasion and modulates cell cycle and apoptosis. a and b Cell migration capacities were detected by wound healing assays after transfected with indicated vectors (magnification, × 50). Scale bar, 200 μm. c and d Cell invasion abilities were determined by transwell assays after transfection (magnification, × 100). Scale bar, 100 μm. e and f The cell cycle progression was analyzed by flow cytometry after transfected with indicated plasmids. g and h Apoptosis rate was analyzed by flow cytometry after downregulation of circAGFG1. i and j Apoptotic cells were assessed by TUNEL (magnification, × 100, scale bar, 100 μm) and Hoechst 33342 (magnification, × 200, scale bar, 50 μm) assays after knockdown of circAGFG1. k The expression levels of apoptosis-related proteins were determined by western blot. Data were showed as mean ± SD, *P < 0.05, **P < 0.01